Identification of novel peptide antagonists for GPIIb/IIIa from a conformationally constrained phage peptide library
- 1 December 1992
- journal article
- research article
- Published by Wiley in Proteins-Structure Function and Bioinformatics
- Vol. 14 (4), 509-515
- https://doi.org/10.1002/prot.340140411
Abstract
Methods have recently been developed to present vast libraries of random peptides on the surface of filamentous phage. To introduce a degree of conformational constraint into random peptides, a library of hexapeptides flanked by cysteine residues (capable of forming cyclic disulfides) was constructed. This library was screened using the platelet glycoprotein, IIb/IIIa, which mediates the aggregation of platelets through binding of fibrinogen. A variety of peptides containing the sequence Arg‐Gly‐Asp or Lys‐Gly‐Asp were discovered and synthesized. The cyclic, disulfide bonded forms of the peptides bound IIb/IIIa with dissociation constants in the nanomolar range, while reduced forms or an analogue in which Ser replaced the Cys residues bound considerably less tightly. These results demonstrate the feasibility for introducing conformational constraints into random peptide libraries and also demonstrates the potential for using phage peptide libraries to discover pharmacologically active lead compounds.This publication has 34 references indexed in Scilit:
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