LOCALIZATION OF PROTEIN-BOUND I131IN THE THYROID GLAND OF THE MOUSE

Abstract

HIGH resolution autoradiography as been used to localize proteinbound I¹³¹ in the thyroid gland of the rat by Leblond and Gross (1948) and by Doniach and Pelc (1949). The theory and methods have been reviewed by Gross, Bogoroch, Nadler and Leblond (1951) and by Fitzgerald, Simmel, Weinstein and Martin (1953). A critical review of the results of autoradiographic studies on the thyroid has been published recently (Doniach, Howard and Pelc, 1953). Two details which appeared to be unsettled were the site of the protein binding of iodine and the significance of the “ring” distribution observed for protein-bound I¹³¹ in the inactive gland. These problems and several others discussed by Leblond and Gross (1948) have now been investigated in the thyroid gland of the mouse. MATERIALS AND METHODS Male C3H mice 3 to 7 months of age weighing 25 to 35 gm. were used. They were fed a pelleted stock diet of moderately low iodine content (Wollman, Scow, Wagner and Morris, 1953.)