Five Hundredfold Overproduction of DNA Ligase After Induction of a Hybrid Lambda Lysogen Constructed in Vitro
- 8 April 1977
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 196 (4286), 188-189
- https://doi.org/10.1126/science.322281
Abstract
A lambda vector that contains the gene for Escherichia coli DNA ligase (lambdagt4-lop-11 lig+) has been modified to achieve overproduction of this enzyme. The third Eco RI site in the lambda chromosome has been altered by mutation, and the left-hand Eco RI fragment has been shortened. The new vector, lambdagt4-lop-11 lig+, forms a stable lysogen which, upon induction, produces a 100-fold increase in DNA ligase activity. Introduction of a phage mutation (S7) that prevents cell lysis results in an even greater increase (500-fold).Keywords
This publication has 12 references indexed in Scilit:
- Characterization of λtrp-transducing bacteriophages made in vitroJournal of Molecular Biology, 1976
- Transposable genetic elements and plasmid evolutionNature, 1976
- Isolation and characterization of a λ specialized transducing phage for the Escherichia coli DNA ligase geneVirology, 1976
- Genetics and function of DNA ligase in Escherichia coliJournal of Molecular Biology, 1973
- Enzymatic Joining of PolynucleotidesPublished by Elsevier ,1970
- Isolation of Pure lac Operon DNA*Nature, 1969
- New mutations in the S cistron of bacteriophage lambda affecting host cell lysisVirology, 1969
- GENETICS OF THE LEFT ARM OF THE CHROMOSOME OF BACTERIOPHAGE LAMBDAGenetics, 1968
- The Integration and Excision of the Bacteriophage Lambda GenomeCold Spring Harbor Symposia on Quantitative Biology, 1968
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951