CO2 Stimulation of the Cornea: A Comparison Between Human Sensation and Nerve Activity in Polymodal Nociceptive Afferents of the Cat

Abstract

Excitation of nociceptors by low pH has been proposed as a cause of pain following tissue injury. Here we have studied the effect of pH reductions caused by application of CO₂ pulses to the cornea on the activity of corneal afferent nerves of the cat and on the magnitude of pain sensations in humans. Single-unit activity was recorded from corneal afferent fibres in anaesthetized cats. The corneal receptive field of A-delta or C polymodal nociceptive units was exposed for 30 s to a gas mixture with different concentrations of CO₂ in air (0, 35, 50, 65, 80 and 98.5%). Responses to CO₂ were evoked at a mean threshold concentration of 40 ± 3% CO₂. They consisted of a discharge of impulses that decayed gradually to a tonic level. In 15% of the units the initial burst was absent. The CO₂ concentration and firing frequency data could be fitted to a power function with an exponent of 1.12. Pulses of CO₂ were also applied to the cornea of 16 human volunteers. Sensations experienced were measured by means of a visual analogue scale and a verbal descriptor scale. Flow was adjusted below the mechanical stimulation threshold (2.8 ± 0.5 mg). When mixtures containing 10-90% CO₂ in 5% steps were applied as 3 s pulses, threshold sensation, described as a mild stinging pain, was evoked at 33.5 ± 4.0% CO₂. This sensation became overtly painful with higher CO₂ concentrations (47.5 ± 3.6% CO₂). For the same subject the sensory threshold was remarkably constant, though it changed with longer exposure times. The relationship between CO₂ concentration and magnitude of pain could be adjusted to a power function with a power exponent of 1.12. Curves of CO₂ concentration versus neural discharges in the cat and versus psychophysical sensation in humans were very similar. These results show that corneal polymodal nociceptors respond to protons, and encode changes in CO₂ concentration presumably reflecting pH changes. The same stimulus evokes corneal pain sensations in humans, thus opening the possibility of using CO₂ as an effective stimulus for corneal aesthesiometry.