Purification and characterization of a membrane-bound ATP diphosphohydrolase from Cicer arietinum (chick-pea)roots
- 1 September 1981
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 197 (3), 637-643
- https://doi.org/10.1042/bj1970637
Abstract
Microsomal membranes from C. arietinum (chick-pea) roots contained an ATP phosphohydrolase activity that could be solubilized by high-ionic-strength media. The enzyme was purified to homogeneity by affinity and ion-exchange chromatography. It has the properties of an ATP diphosphohydrolase (apyrase, EC 3.6.1.5) that hydrolyzes different nucleoside di- and triphosphates but has no activity towards monophosphoric esters and pyrophosphate. No stimulation by K+ could be demonstrated for either the membrane-bound or the purified enzyme, and therefore it would seem not to be related to the K+-dependent ATPase postulated to mediate K+ transport in plants.This publication has 26 references indexed in Scilit:
- Solubilization and Partial Purification of the Adenosine Triphosphatase from a Corn Root Plasma Membrane FractionPlant Physiology, 1980
- Purification and Characterization of a Cation-stimulated Adenosine Triphosphatase from Corn RootsPlant Physiology, 1978
- Purification of Plasma Membranes from Roots of BarleyPlant Physiology, 1978
- Adenosine Triphosphatase from Soybean Callus and Root CellsPlant Physiology, 1977
- Cation-stimulated Adenosine Triphosphatase Activity and Cation Transport in Corn RootsPlant Physiology, 1976
- Association of Latent Cellulase Activity with Plasma Membranes from Kidney Bean Abscission ZonesPlant Physiology, 1976
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- Characterization of Plasma Membrane-associated Adenosine Triphosphase Activity of Oat RootsPlant Physiology, 1973
- Electrophoretic analysis of the major polypeptides of the human erythrocyte membraneBiochemistry, 1971
- Triphosphopyridine nucleotide: cytochrome C reductase of Saccharomyces Cerevisiae a “microsomal” enzymeBiochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects, 1964