Highly selective affinity labelling of RNA polymerase B (II) from wheat germ
- 12 May 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 200 (2), 287-290
- https://doi.org/10.1016/0014-5793(86)81154-1
Abstract
DNA-dependent RNA polymerase B (II) from wheat germ was modified by incubation with 4-[N-(β-hydroxyethyl)-N-methyl]benzaldehyde esters of AMP, ADP or ATP, followed by reduction with NaBH4. Reaction of the modified enzyme with [α-32P]UTP in the presence of various DNA templates led to a highly selective affinity labelling of the subunit with M r 140000 by covalently linked ApU. Labelling was inhibited by 1 α-amanitin.
Keywords
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