Serum Factor Supporting Long-Rerm Survival of Rat Central Neurons in Culture
- 24 June 1983
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 220 (4604), 1394-1396
- https://doi.org/10.1126/science.6857258
Abstract
Gel filtration of serum at pH 3.6 yielded a fraction that supported long-term (months) survival of dissociated rat central neurons in monolayer culture more reliably than the traditionally used unfractionated serum. The cultures remained neuron-rich, because this fraction did not support the proliferation of glia and fibroblasts that occurs in whole serum. With an apparent molecular weight of 55,000 and an isoelectric point of 5.6, the active factor (or factors) in this fraction is distinct from any well-defined growth factor.Keywords
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