A Simple Polymerase Chain Reaction-Based Method for the Construction of Recombinase-Mediated Cassette Exchange Donor Vectors
- 1 November 2008
- journal article
- Published by Oxford University Press (OUP) in Genetics
- Vol. 180 (3), 1763-1766
- https://doi.org/10.1534/genetics.108.094508
Abstract
Here we describe a simple method for generating donor vectors suitable for targeted transgenesis via recombinase-mediated cassette exchange (RMCE) using the ΦC31 integrase. This PCR-based strategy employs small attB “tails” on the primers used to amplify a sequence of interest, permitting the rapid creation of transgenes for in vivo analysis.Keywords
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