Simultaneous autoradiographic and histochemical analysis of bone formed in vitro.
Open Access
- 1 June 1986
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 34 (6), 769-773
- https://doi.org/10.1177/34.6.3701035
Abstract
The simultaneous histochemical demonstration of alkaline phosphatase activity and autoradiographic demonstration of [3H]-thymidine uptake is valuable for study of bone cell kinetics in vivo or in vitro. By use of this technique, it has been possible to detect changes induced by a single dose of dexamethasone (10(-7) M) in the time course of alkaline phosphatase activity, the number of alkaline phosphatase-positive cells, and [3H]-thymidine labeling in bone formed in vitro.This publication has 11 references indexed in Scilit:
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