Inhibition of restriction endonuclease cleavage via triple helix formation by homopyrimidine oligonucleotides
- 12 December 1989
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 28 (25), 9617-9619
- https://doi.org/10.1021/bi00451a011
Abstract
A 17-mer homopyrimidine oligonucleotide was designed to bind to the major groove of SV40 DNA at a 17 base pair homopurine-homopyrimidine sequence via Hoogsteen base pairing. This sequence contains the recognition site for the class II-S restriction enzyme Ksp 632-I. The oligonucleotide was shown to inhibit enzymatic cleavage under conditions that allow for triple helix formation. Inhibition is sequence-specific and occurs in the micromolar concentration range. Triple helix formation by oligonucleotides opens new possibilities for sequence-specific regulation of gene expression.This publication has 4 references indexed in Scilit:
- Site-Specific Oligonucleotide Binding Represses Transcription of the Human c- myc Gene in VitroScience, 1988
- Ksp632I, a novel class-IIS restriction endonuclease from Kluyvera sp. strain 632 with the asymmetric hexanucleotide recognition sequence: 5'-CTCTTC(N)1-3' 3'-GAGAAG(N)4-5'Gene, 1988
- A stable complex between homopyrimidine oligomers and the homologous regions of duplex DNAsNucleic Acids Research, 1988
- Construction and characterization of new cloning vehicles IV. Deletion derivatives of pBR322 and pBR325Gene, 1980