Characteristics of chloroplast thylakoid lipid composition associated with resistance to triazine herbicides

Abstract

A detailed comparison of the polar-lipid composition of chloroplast thylakoid membranes isolated from triazine-susceptible and triazine-resistant biotypes of Chenopodium album, Senecio vulgaris, Poa annua and Amaranthus retroflexus has been carried out. No major differences in the composition of the bulk lipid matrix were found except for a slightly higher monogalactosyldiacylglycerol to digalactosyldiacylglycerol ratio in resistant compared with susceptible biotypes. There was, however, in the case of resistant plants a higher level of phosphatidylglycerol-containing transhexadecenoic acid in membrane fractions enriched in photosystem two. It is concluded that although the minor differences could contribute to triazine resistance it is more likely that they reflect secondary alterations in membrane organisation associated with changes in relative levels of pigment-protein complexes.