Intraneuronal guanylyl-imidodiphosphate injection mimics long-term synaptic hyperpolarization in Aplysia.
- 1 December 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (12), 4689-4692
- https://doi.org/10.1073/pnas.73.12.4689
Abstract
The phosphodiesterase (3'':5''-cyclic[c]AMP 5''-nucleotidohydrolase, EC 3.1.4.17) inhibitor theophylline enhances both the amplitude and duration of a long-lasting synaptic hyperpolarization in identified neuron R15 in A. californica. Intraneuronal injection into R15 of guanylyl-imidodiphosphate, an adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] activator, results in a deep and long-lasting hyperpolarization of the cell, similar to that produced by synaptic stimulation. Biochemical analysis confirms that guanylyl-imidodiphosphate activates adenylate cyclase in A. californica nervous tissue, without affecting phosphodiesterase activity. Probably, cAMP plays a role in long-lasting synaptic inhibition and a post-synaptic site of action for cAMP is present.This publication has 19 references indexed in Scilit:
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