Adenosine Triphosphate-Linked Control of Pseudomonas aeruginosa Glucose-6-Phosphate Dehydrogenase
- 1 April 1967
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 93 (4), 1337-+
- https://doi.org/10.1128/jb.93.4.1337-1345.1967
Abstract
Extracts of P. aeruginosa (ATCC 7700) cells grown on glucose, gluconate, or glycerol had enzyme activities related to the Entner-Doudoroff pathway. These activities were present in no more than trace amounts when the bacteria were grown on succinate. Fructose-1, 6-diphosphate aldolase could not be detected in extracts of the bacteria grown on any of the above carbon sources. Therefore, it appears that P. aeruginosa degrades glucose via in inducible Entner-Doudoroff pathway. The apparent absence of fructose-l,6-diphosphate aldolase in cells growing on succinate suggests that the bacteria can form hexose and pentose phosphates from succinate by an alternate route. D-Glucose-6-phosphate dehydrogenase, a branch-point enzyme of the Entner-Doudoroff pathway, was purified 50-fold from glucose-grown cells. Its molecular weight, estimated by sucrose density gradient centrifugation, was found to be approximately 190,000. The enzyme was strongly inhibited by adenosine triphosphate, guanosine triphosphate, and deoxyguanosine triphosphate, which decreased the apparent binding of glucose-6-phosphate to the enzyme. It is suggested that adenine nucleotide-linked control of glucose-6-phosphate dehydrogenase may regulate the overall catabolism of hexose phosphates and prevent their wasteful degradation under certain conditions requiring gluconeogenesis.This publication has 20 references indexed in Scilit:
- Biosynthesis of Ribose and DeoxyribosePublished by Wiley ,2006
- The Aerobic Pseudomonads a Taxonomic StudyJournal of General Microbiology, 1966
- Biological Feedback Control at the Molecular LevelScience, 1965
- Kinetics of regulatory enzymes: Effectors for yeast phosphofructokinase do not alter the apparent kinetic order of the reactionBiochemical and Biophysical Research Communications, 1965
- Energy-linked regulation of threonine metabolism by ADPBiochemical and Biophysical Research Communications, 1963
- Use of chloramphenicol to study control of RNA synthesis in bacteriaBiochimica et Biophysica Acta, 1961
- A Method for Determining the Sedimentation Behavior of Enzymes: Application to Protein MixturesJournal of Biological Chemistry, 1961
- Catabolite RepressionCold Spring Harbor Symposia on Quantitative Biology, 1961
- The catabolism of glucose and gluconate in Pseudomonas speciesArchives of Biochemistry and Biophysics, 1959
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951