Vernetzung wäßriger Alginsäure mittels der Vierkomponenten-Kondensation unter Einschluß-Immobilisierung von Enzymen / Crosslinking of Aqueous Alginic Acid by Four Component Condensation with Inclusion Immobilization of Enzymes

Abstract

The four component condensation (4 CC, Ugi reaction) of aqueous alginic acid with formaldehyde, difunctional amine and isocyanide components produces a gel. Enzymes, e.g. “acidic phosphatase” (AP) and L-(+)-lactate dehydrogenase (LDH), can be immobilized by inclusion in the above crosslinked alginic acid. The enzymes retain some of their activity (LDH: 8-10%; AP: 82-87%).