Insertion Mutagenesis of Embryonal Carcinoma Cells by Retroviruses
- 3 May 1985
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 228 (4699), 554-558
- https://doi.org/10.1126/science.3838595
Abstract
Mutagenesis was studied in cultured F9 embryonal carcinoma cells infected with a variant of Moloney murine leukemia virus. Proviral insertion induced the inactivation of the hypoxanthine phosphoribosyltransferase locus, and the virus was used to isolate the mutated genes rapidly. Mutagenesis by these methods may be useful for the genetic dissection of the various mammalian cell phenotypes.Keywords
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