Twist negatively regulates osteoblastic differentiation in human periodontal ligament cells
- 3 August 2006
- journal article
- research article
- Published by Wiley in Journal of Cellular Biochemistry
- Vol. 100 (2), 303-314
- https://doi.org/10.1002/jcb.21038
Abstract
Periodontal ligament (PDL) is a thin fibrous connective tissue located between two mineralized tissues, alveolar bone and cementum, which maintains a constant width physiologically. The mechanisms by which PDL resists mineralization are not well understood. Twist is a basic helix loop helix protein that plays a central role in regulation of early osteogenesis. We investigated the localization of Twist in PDL and compared the expression of Twist and osteoblast-related genes in PDL cells with those in osteoblast-like cells in the presence or absence of recombinant human bone morphogenetic protein (BMP)-2. Histochemical analysis showed that Twist was expressed along alveolar bone surface in PDL. PDL cells constitutively expressed Twist gene and the expression level was higher than that in osteoblast-like cells. In osteoblast-like cell culture, BMP-2 enhanced osteoblast-related gene expression, while Twist expression was slightly decreased. In contrast, BMP-2 increased runt-related transcription factor (Runx)-2, but failed to enhance alkaline phosphatase (ALP) and osteocalcin (OCN) gene expression in PDL cells. Interestingly, unlike in osteoblast-like cells, Twist expression was upregulated by BMP-2 in PDL cells. We transiently knocked down Twist gene in PDL cells using a short interference RNA expression vector (siTwist) and found that ALP, osteopontin (OPN), bone sialoprotein (BSP) genes expression and basal level of ALP activity were slightly increased, whereas Runx2 and OCN genes were not affected. Collectively, these results suggest that Twist may act as a negative regulator of osteoblastic differentiation in PDL cells. J. Cell. Biochem. 100: 303–314, 2007.Keywords
This publication has 47 references indexed in Scilit:
- Role of Ascorbic Acid in Periodontal Ligament Cell DifferentiationThe Journal of Periodontology, 2004
- MyoD enhances BMP7-induced osteogenic differentiation of myogenic cell culturesJournal of Cell Science, 2004
- A cell line with characteristics of the periodontal ligament fibroblasts is negatively regulated for mineralization and Runx2/Cbfa1/Osf2 activity, part of which can be overcome by bone morphogenetic protein-2Journal of Cell Science, 2002
- Effects of Occlusal Loading on Ankylosis, Bone, and Cementum Formation During Bone Morphogenetic Protein‐2‐Stimulated Periodontal Regeneration In VivoThe Journal of Periodontology, 1999
- Mutations within or upstream of the basic helix–loop–helix domain of the TWIST gene are specific to Saethre-Chotzen syndromeEuropean Journal of Human Genetics, 1999
- Induction of new bone by ceramic bovine bone with recombinant human bone morphogenetic protein 2 and transforming growth factor ßInternational Journal of Oral & Maxillofacial Surgery, 1998
- Bone morphogenetic protein-2 does not alter the differentiation pathway of committed progenitors of osteoblasts and chondroblastsCell and tissue research, 1996
- Human Periodontal Cells Initiate Mineral‐Like Nodules In VitroThe Journal of Periodontology, 1991
- The non-osteogenic mouse pluripotent cell line, C3H10T1/2, is induced to differentiate into osteoblastic cells by recombinant human bone morphogenetic protein-2Biochemical and Biophysical Research Communications, 1990
- Ectopic Bone Induction on and in Porous Hydroxyapatite Combined with Collagen and Bone Morphogenetic ProteinClinical Orthopaedics and Related Research, 1988