Alteration of the cell membrane architecture during suspension and monolayer culturing
- 15 March 1976
- journal article
- research article
- Published by Wiley in International Journal of Cancer
- Vol. 17 (3), 407-415
- https://doi.org/10.1002/ijc.2910170318
Abstract
Using suspension‐growth‐adapted HeLa (S3) cells, we followed the alterations in cell morphology during attachment and spreading on a glass surface in vitro by scanning electron microscopy. Suspension cultivation obviated the need for trypsinization or EDTA treatment of the cells before seeding, thus the cell surface was not chemically altered. The suspension‐grown cells were spherical and covered with microvilli. Three hours after seeding into stationary culture, attached cells had surface blebs and large processes, but no microvilli. During the following 9 h, the surface topography altered stepwise producing a complex lamelloplasm. The paper discusses how these membrane events are related to the cell cycle and metastasis.This publication has 19 references indexed in Scilit:
- THE UPPER CELL SURFACE: ITS INABILITY TO SUPPORT ACTIVE CELL MOVEMENT IN CULTUREThe Journal of cell biology, 1974
- Some speculations on the rate of adhesion of cells to coverslipsJournal of Theoretical Biology, 1972
- Lipid Bilayers and BiomembranesAnnual Review of Biochemistry, 1972
- Local inhibition of ruffling during contact inhibition of cell movementExperimental Cell Research, 1971
- The occurrence of microvilli during spreading and growth of BHK21/C13 fibroblastsExperimental Cell Research, 1970
- Ultrastructure and Physiology of PollenAnnual Review of Plant Physiology, 1968
- The movements of fibrocytesExperimental Cell Research, 1961
- Cell Contacts: Some Physical ConsiderationsThe American Naturalist, 1960
- Interference microscope studies of cell contacts in tissue cultureExperimental Cell Research, 1958
- Micro-operations on cells in tissue culturesProceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character, 1931