I-Rel: a novel rel-related protein that inhibits NF-kappa B transcriptional activity.
- 1 May 1992
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 6 (5), 745-760
- https://doi.org/10.1101/gad.6.5.745
Abstract
The NF-kappa B transcription factor complex is comprised of two subunits, p50 and p65, that share significant homology to the rel oncogene. We have isolated a cDNA encoding a novel 66-kD rel-related protein, designated I-Rel. Unlike other rel-related proteins, I-Rel does not interact with DNA. I-Rel forms heterodimers with p50, however, and greatly attenuates its DNA-binding activity--an effect probably resulting from the presence of a domain inhibitory to DNA binding present within the 121 amino-terminal residues of I-Rel. In contrast, I-Rel does not associate with p65. Transfection experiments demonstrate that I-Rel suppresses NF-kappa B-induced transcription, probably through its association with p50. Expression of I-Rel mRNA is induced by mitogenic stimulation and accumulates after the appearance of p50 transcripts. Our findings suggest that p50 and I-Rel are components of a feedback pathway where expression of I-Rel may modulate indirectly the expression of genes responsive to the NF-kappa B transcription factor complex.Keywords
This publication has 53 references indexed in Scilit:
- Rel-Associated pp40: an Inhibitor of the Rel Family of Transcription FactorsScience, 1991
- Isolation of a rel -related Human cDNAThat Potentially Encodes the 65-kD Subunit of NF-κBScience, 1991
- DNA binding and IκB inhibition of the cloned p65 subunit of NF-κB, a rel-related polypeptideCell, 1991
- The v-rel oncogene encodes a κB enhancer binding protein that inhibits NF-κB functionCell, 1990
- Cloning of the p50 DNA binding subunit of NF-κB: Homology to rel and dorsalCell, 1990
- The DNA binding subunit of NF-κB is identical to factor KBF1 and homologous to the rel oncogene productCell, 1990
- Functional Analysis of CAR, the Target Sequence for the Rev Protein of HIV-1Science, 1989
- The same inducible nuclear proteins regulates mitogen activation of both the interleukin-2 receptor-alpha gene and type 1 HIVCell, 1988
- GAL4 activates gene expression in mammalian cellsCell, 1988
- The location of cis-acting regulatory sequences in the human T cell lymphotropic virus type III (HTLV-III/LAV) long terminal repeatCell, 1985