Analysis of Peptide Secondary Structures by Photoactivatable Amino Acid Analogues
- 26 October 2012
- journal article
- research article
- Published by Wiley in Angewandte Chemie International Edition
- Vol. 51 (50), 12602-12605
- https://doi.org/10.1002/anie.201205308
Abstract
Photochemical cross-linking was applied to trap intramolecular interactions in peptides. The incorporation of diazirine-labeled amino acid analogues in combination with high-resolution mass spectrometry made it possible to catch reverse-turn conformations within peptides, exactly map their self-interacting surfaces, and discriminate between stable and transient interactions.Keywords
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