Simple Method for the Collection of Pancreatic Islets by the Use of Ficoll-Conray Gradient

Abstract

This method involves collagenase digestion of the [rat] pancreas followed by the use of Ficoll-Conray discontinuous gradient for the separation of isolated islets from unwanted acinal debris. Ficoll-Conray A solution was prepared by mixing undialyzed 12.5% Ficoll and 33.4% Conray in the ratio 2:1, to make a specific gravity of 1.095 and osmolarity of 396 mOsm[osmoles]/l. The solution B, C, and D, with specific gravities 1.084, 1.072, and 1.048, respectively, were obtained by diluting A solution with distilled water. Using Ficoll-Conray gradient, about 200 viable islets which maintained excellently their morphology and function, were collected consistently from a young adult rat pancreas.