The Effect of Chloramphenicol on Growth and Mitochondrial Structure of Pythium ultimum

Abstract

Chloramphenicol inhibited the growth of P. ultimum, showing a maximum dose-response at a concentration of 100 [mu]g/ml. Mycelium grown in the presence of chloramphenicol 100 [mu]g/ml., was devoid of the mitochondrial cytochromes aa3 and b but contained increased cytoplasmic cvtochrome c. Mitochondria in thin sections of hyphae grown in the presence of chloramphenicol resembled mitochondria in normal hyphae. In negatively stained preparations of isolated normal mitochondira stalked particles were observed on the cristae. These particles could not be demonstrated in mitochondria from hyphae grown in the presence of chloramphenicol 100 [mu]g/ml.