Early Antihepatitis C Virus Response with Second—Generation C200/C22 ELISA
- 1 May 1992
- journal article
- research article
- Published by Wiley in Vox Sanguinis
- Vol. 62 (4), 208-212
- https://doi.org/10.1111/j.1423-0410.1992.tb01200.x
Abstract
Detection of early antibody to hepatitis C virus (HCV) by a new second‐generation C200/C22 anti‐HCV enzyme‐linked immunosorbent assay (ELISA) and a four‐antigen recombinant immunoblot assay (4‐RIBA) was compared with the first‐generation anti‐HCV C100 ELISA using sequential serum samples of 9 recipients who were infected with HCV, as detected by polymerase chain reaction after transfusion of blood products. Within 26 weeks after transfusion, 9/9 (100%) recipients seroconverted with C200/22 ELISA, and 6/9 (67%) seroconverted with C100 ELISA. Compared with C100 ELISA, C200/C22 ELISA seroconversion occurred simultaneously in 3 cases, 5–6 weeks earlier in 3 other cases, and 20 weeks earlier in 1 case. Seven of 9 (78%) recipients became positive, and 2/9 (22%) became indeterminate with 4‐RIBA. In 8 cases with clinical posttransfusion hepatitis non‐A, non‐B (PTH‐NANB), anti‐HCV C200/C22 ELISA seroconversion occurred 2–17 (mean 6) weeks after the onset of hepatitis. In 6 cases of PTH‐NANB, anti‐HCV C100 ELISA seroconversion occurred 2–26 (mean 9) weeks after the onset of hepatitis. It is concluded that the second‐generation C200/C22 ELISA is more sensitive than the C100 ELISA for the detection of antibody during early HCV infection. Indeterminate 4‐RIBA results are found in the early phase of HCV infection.Keywords
This publication has 11 references indexed in Scilit:
- Second-generation RIBA to confirm diagnosis of HCV infectionThe Lancet, 1991
- Genetic organization and diversity of the hepatitis C virus.Proceedings of the National Academy of Sciences, 1991
- Characterization of the terminal regions of hepatitis C viral RNA: identification of conserved sequences in the 5' untranslated region and poly(A) tails at the 3' end.Proceedings of the National Academy of Sciences, 1991
- Confirmation of hepatitis C virus infection by new four-antigen recombinant immunoblot assayThe Lancet, 1991
- Recombinant immunoblot assay for hepatitis C virus antibody as predictor of infectivityThe Lancet, 1990
- Infectivity of blood seropositive for hepatitis C virus antibodiesThe Lancet, 1990
- Detection of hepatitis C viral sequences in non-A, non-B hepatitisThe Lancet, 1990
- Detection of Antibody to Hepatitis C Virus in Prospectively Followed Transfusion Recipients with Acute and Chronic Non-A, Non-B HepatitisNew England Journal of Medicine, 1989
- HEPATITIS C VIRUS ANTIBODIES AMONG RISK GROUPS IN SPAINThe Lancet, 1989
- An Assay for Circulating Antibodies to a Major Etiologic Virus of Human Non-A, Non-B HepatitisScience, 1989