THE IMMUNODEPRESSIVE AND HEMATOTOXIC ACTIVITY OF IMIDAZOLE-4-CARBOXAMIDE,5-(3,3-DIMETHYL-1-TRIAZENO) IN MICE

Abstract

The immunodepressive and bone marrow stem cell-reducing activities of imidazole-4-carboxamide,5-(3,3-dimethyl-1-triazeno) (DTIC), an antitumoral agent reported to possess little immunodepressive capacity in man, were investigated in mice and compared with those displayed by cyclophosphamide (Cy). In this species, single doses of DTIC could profoundly depress antilymphoma allograft resistance as well as the number of antibody-producing cells after primary and secondary stimulation with sheep erythrocytes. The highest immunodepressive activity was observed when DTIC was given before antigen, and the effect observed was substantially more long lasting than seen with Cy, which was significantly more active on a milligram per kilogram basis. Both agents displayed a quantitatively equal activity in reducing bone marrow stem cells. DTIC can be an effective immunodepressant. This activity may have clinical implications.