Amino Acid Incorporation by Wheat Chloroplasts
- 1 May 1966
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 41 (5), 764-770
- https://doi.org/10.1104/pp.41.5.764
Abstract
Isolated chloroplasts from wheat leaves incorporated radioactive amino acids into proteins. Both physiological and biochemical evidence showed that contaminating bacteria were not responsible for this activity. Activity was best in plastids from 5-day-old or younger seedlings; a sharp drop usually occurred by day 6 or 7. The system required added adenosine triphosphate, guanosine triphosphate, and Mg ions, and was inhibited by ribonuclease, puromycin, and chloramphenicol. Preliminary evidence is presented that polyribo-somes were present in the young leaf chloroplast fraction. Half the protein formed in a 20-minute incubation was released in soluble form.Keywords
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