Isolation and primary structure of novel neurointermediate pituitary peptides derived from the C-terminal of the rat vasopressin-neurophysin precursor (propressophysin)

Abstract

Four novel peptides were isolated from rat neurointermediate lobes by gel filtration and high-pressure liquid chromatography. Analyses of amino acid composition and sequence showed that all four peptides were derived from the C-terminal portion of propressophysin (CPP); they were identified as the glycopeptides CPP 1–19, CPP 1–20, CPP 22–37 and CPP 22–39. Processing of CPP 1–39 could thus produce the four isolated peptides by specific post-Arg or post-Leu cleavages.