High Rate of MCR-1–Producing Escherichia coli and Klebsiella pneumoniae among Pigs, Portugal
Open Access
- 1 December 2017
- journal article
- Published by Centers for Disease Control and Prevention (CDC) in Emerging Infectious Diseases
- Vol. 23 (12), 2023-2029
- https://doi.org/10.3201/eid2312.170883
Abstract
The mcr-1 (mobile colistin resistance 1) gene, which encodes phosphoethanolamine transferase, has been recently identified as a source of acquired resistance to polymyxins in Escherichia coli. Using the SuperPolymyxin selective medium, we prospectively screened 100 pigs at 2 farms in Portugal for polymyxin-resistant Enterobacteriaceae and recovered 98 plasmid-mediated MCR-1–producing isolates. Most isolates corresponded to nonclonally related E. coli belonging to many sequence types; we also found 2 Klebsiella pneumoniae sequence types. The mcr-1 gene was carried on IncHI2 or IncP plasmid backbones. Our finding of a high rate of MCR-1 producers on 2 pig farms in Portugal highlights the diffusion of that colistin-resistance determinant at the farm level. The fact that the pigs received colistin as metaphylaxis in their feed during the 6 weeks before sampling suggests selective pressure.Keywords
This publication has 44 references indexed in Scilit:
- Plasmid-mediated carbapenem and colistin resistance in a clinical isolate of Escherichia coliThe Lancet Infectious Diseases, 2016
- Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological studyThe Lancet Infectious Diseases, 2015
- Use of colistin-containing products within the European Union and European Economic Area (EU/EEA): development of resistance in animals and possible impact on human and animal healthInternational Journal of Antimicrobial Agents, 2015
- The Clermont Escherichia coli phylo‐typing method revisited: improvement of specificity and detection of new phylo‐groupsEnvironmental Microbiology Reports, 2012
- Moraxella porci sp. nov., isolated from pigsInternational Journal of Systematic and Evolutionary Microbiology, 2010
- Identification of plasmids by PCR-based replicon typingJournal of Microbiological Methods, 2005
- Prevalence of Derepressed AmpC Mutants and Extended-Spectrum β-Lactamase Producers among Clinical Isolates of Citrobacter freundii , Enterobacter spp., and Serratia marcescens in Korea: Dissemination of CTX-M-3, TEM-52, and SHV-12Journal of Clinical Microbiology, 2005
- Diversity of genetic environment ofblaCTX-MgenesFEMS Microbiology Letters, 2004
- Diversity of genetic environment of blaCTX-M genesFEMS Microbiology Letters, 2004
- Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coliPlasmid, 1984