Phosphohistidine is found in basic nuclear proteins ofPhysarum polycephalum
- 19 October 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 239 (1), 151-154
- https://doi.org/10.1016/0014-5793(88)80563-5
Abstract
Nuclear extracts of the true slime mold, Physarum polycephalum, show protein histidine kinase activity towards exogenous histones [(1985) J. Biol. Chem. 260, 16106–16113]. Physarum microplasmodia were labeled with [32P]phosphate in vivo and two basic proteins containing alkali-stable phosphate were detected. The labeled proteins comigrated with Physarum histones H1 (approximately) and H2A and phosphoamino acid analysis showed that each protein contained [32P]phosphohistidine. The H2A-like protein was also labeled in isolated nuclei incubated with [35S]thio-ATP. We conclude that some Physarum nuclear proteins contain phosphohistidineKeywords
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