Proteins in chromosome banding
- 1 June 1980
- journal article
- research article
- Published by Springer Nature in Chromosoma
- Vol. 79 (1), 29-41
- https://doi.org/10.1007/bf00328470
Abstract
Nuclei were isolated from Chinese hamster cells, treated with hypotonic KCl, fixed in acetic methanol, and either air-dried in glass tubes (in situ) or left in suspension (in vitro). These preparations were then exposed to a variety of G-banding treatments, including the 2 × SSC, urea, NaCl-urea, and trypsin methods. The proteins extracted into the treatment solution and those remaining in the nuclei were analyzed by SDS polyacrylamide gel electrophoresis. The three former treatments extracted specific subsets of the total nuclear nonhistone proteins into the treatment solution. Some of the extracted nonhistones were common to all treatments while others were unique to a particular treatment. Variable amounts and types of the histones were also extracted by these treatments, but significant quantities of all of these proteins still remained in the nuclei afterwards. The trypsin treatment appeared to degrade some of the nonhistones, while other non-histones, as well as the histones, were relatively resistant to trypsin digestion. Although there were a few differences in the residual proteins found in the nuclei after the various G-band treatments, the overall electrophoretic patterns of these proteins were generally similar. The results indicate that the G-banding techniques induce specific and reproducible changes in the proteins of isolated nuclei. If these banding treatments induce similar changes in the proteins of mitotic chromosomes, such alterations might be involved in mechanisms of chromosome banding.Keywords
This publication has 26 references indexed in Scilit:
- DNA-protein interactions and chromosome bandingExperimental Cell Research, 1977
- Histones removed by fixationExperimental Cell Research, 1977
- Histones and G Banding of ChromosomesScience, 1976
- Uneven extraction of protein in Chinese hamster chromosomes during G-staining proceduresExperimental Cell Research, 1976
- Histone distribution in chromosomes revealed by antihistone seraExperimental Cell Research, 1976
- The Possible Role of Histones in the Mechanism of Chromosomal G BandingScience, 1975
- Histones in fixed cytological preparations of Chinese hamster chromosomes demonstrated by immunofluorescenceNature, 1975
- Mechanisms of chromosome banding: II. Evidence that histones are not involvedExperimental Cell Research, 1974
- Chromosomal proteins in fixed metaphase cellsHistochemistry and Cell Biology, 1974
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970