Molecular cloning, functional characterization and mRNA expression analysis of the murine chemokine receptor CCR6 and its specific ligand MIP‐3α1
- 27 November 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 440 (1-2), 188-194
- https://doi.org/10.1016/s0014-5793(98)01450-1
Abstract
We have cloned the murine CCR6 receptor and its ligand, the β-chemokine mMIP-3α. Calcium mobilization assays performed with mCCR6 transfectants showed significant responses upon addition of mMIP-3α. Murine MIP-3α RNA is expressed in thymus, small intestine and colon, whereas mCCR6 RNA is expressed in spleen and lymph nodes. RT-PCR analysis of FACS-sorted lymphoid and antigen presenting cell subsets showed mCCR6 expression mainly in B cells, CD8− splenic dendritic cells and CD4+ T cells. The cloning and functional characterization of the mCCR6 and mMIP-3α will allow the study of the role of these proteins in mouse models of inflammation and immunityKeywords
This publication has 37 references indexed in Scilit:
- Dendritic Cell Development: Multiple Pathways to Nature's AdjuvantsThe International Journal of Cell Cloning, 1997
- CCR6, a CC Chemokine Receptor that Interacts with Macrophage Inflammatory Protein 3α and Is Highly Expressed in Human Dendritic CellsThe Journal of Experimental Medicine, 1997
- STRL22 Is a Receptor for the CC Chemokine MIP-3αBiochemical and Biophysical Research Communications, 1997
- Proteinase-activated receptors: structural requirements for activity, receptor cross-reactivity, and receptor selectivity of receptor-activating peptidesCanadian Journal of Physiology and Pharmacology, 1997
- Molecular Cloning of a Novel Human CC Chemokine Liver and Activation-regulated Chemokine (LARC) Expressed in LiverJournal of Biological Chemistry, 1997
- A new class of membrane-bound chemokine with a CX3C motifNature, 1997
- Molecular cloning and chromosomal mapping of a novel human gene, ChemR1, expressed in T lymphocytes and polymorphonuclear cells and encoding a putative chemokine receptorEuropean Journal of Immunology, 1996
- Molecular Cloning and RNA Expression of Two New Human Chemokine Receptor-like GenesBiochemical and Biophysical Research Communications, 1996
- Lymphotactin: a Cytokine that Represents a New Class of ChemokineScience, 1994
- Human and murine dermis contain dendritic cells. Isolation by means of a novel method and phenotypical and functional characterization.Journal of Clinical Investigation, 1993