The Subcellular Distribution and State of the Elongation Factor Tu in Extracts of Escherichia coli B
- 1 May 1976
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 64 (2), 597-606
- https://doi.org/10.1111/j.1432-1033.1976.tb10339.x
Abstract
The concentration of elongation factor Tu(EF-Tu) is about 8 times that of ribosomes in extracts of E. coli B grown in glucose-minimal medium. Of the EF-Tu, 90% is found in the ribosome-free fraction of the cell and 75% of this is free of other macromolecules as judged by chromatography on Sephadex G-100; about 10% is bound to the elongation factor EF-Ts and the remaining 10-15% is eluted from Sephadex G-100 earlier than expected for its MW. Tryptic peptide maps of the 3 forms of EF-Tu are essentially indistinguishable.This publication has 23 references indexed in Scilit:
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