THE CULTIVATION FROM GRANULOMA INGUINALE OF A MICROORGANISM HAVING THE CHARACTERISTICS OF DONOVAN BODIES IN THE YOLK SAC OF CHICK EMBRYOS

Abstract

Two cystine agar slant smears of human tissue rich in Donovan bodies, incubated at 37[degree]C for 96 hrs., appeared free of bacteria. Saline washings from these slants were inoculated into the yolk of living chick embryos. Subsequent smears revealed microorganisms with all morphological characteristics of Donovan bodies. The organisms were neither cultivable on ordinary media nor pathogenic for mice, dogs or monkeys.