Vital Staining with Neutral Red and Trypan Blue of3H-Thymidine-Labeled Cells Prior to Autoradiography
- 1 January 1967
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 42 (3), 143-146
- https://doi.org/10.3109/10520296709114997
Abstract
Mouse cells cultivated in vitro were continuously labeled with tritlated thymidine for 48 hr. In representative groups of labeled cells, the percentages of dead cells were obtained by vital staining with either neutral red or trypan blue. After fixation and autoradiography, the fraction of nonlabeled cells was determined in the same group of cells which had been used for interpretation of viability. Neither neutral red nor trypan blue used prior to autoradiography caused spurious grain formation in the emulsion.This publication has 3 references indexed in Scilit:
- Stains Compatible with Dipping RadioautographyStain Technology, 1963
- Cell proliferation and migration as revealed by radioautography after injection of thymidine‐H3 into male rats and miceJournal of Anatomy, 1960