A METHOD FOR THE SEPARATION AND ESTIMATION OF NEUTRAL STEROIDS IN THE URINE OF NEWBORN INFANTS
- 1 August 1963
- journal article
- research article
- Published by Bioscientifica in Journal of Endocrinology
- Vol. 27 (1), 31-51
- https://doi.org/10.1677/joe.0.0270031
Abstract
SUMMARY: A method is described for the separation and estimation of neutral steroids in infant urine. The free steroids are obtained by a two-stage enzyme hydrolysis. Following preliminary chromatography, the extracts containing steroids of low and medium polarity are suitable for final resolution. The extract containing steroids of high polarity requires further purification by partition between ether-ethanol and aqueous ammonium sulphate, followed by reversed-phase paper chromatography. Final resolution of steroids in fractions of different polarity is achieved using Bush aqueous methanol systems. Losses incurred in the method are estimated by the use of 4-14C steroids.Keywords
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