Photosensitive nitrile hydratase intrinsically possesses nitric oxide bound to the non‐heme iron center: evidence by Fourier transform infrared spectroscopy
- 16 January 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 358 (1), 9-12
- https://doi.org/10.1016/0014-5793(94)01374-a
Abstract
Nitrile hydratase (NHase) from Rhodococcus sp. N771 is a photosensitive enzyme that catalyzes hydration of nitriles to the corresponding amides. Light-induced Fourier transform infrared difference spectra between the inactive and active forms of NHase were measured with both the natural (14N) and 15N-labeled NHases. The results showed, for the first time, that NHase intrinsically possesses nitric oxide (NO) molecules bound to the non-heme iron center. The possible role of NO in the photoactivation process of NHase is discussedKeywords
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