Über die Darstellung von Methämoglobin, über Fluorhämoglobin, über Papainspaltung von Hämoglobin und über das Hämoglobin bei perniziöser Anämie.

Abstract

Correcting previous reports, crystalline methemoglobin is easily and uniformly made by the treatment of horse oxyhemoglobin with 5% alcohol at 37-40[degree]. The conversion of methemoglobin into its fluor-derivative requires a maximum of 30 min. The absorption band lies in the red area (606 m[mu] [plus or minus] 1). Papain-HCN at pH 5 acts similarly to activated trypsin at pH 9 on blood pigment, producing a hemin-proteose. The hemoglobin in pernicious anemia is of the normal, adult type, and does not react to alkalies as does that of the newborn.