Differences Among Monoclonal Antibodies to Barley Yellow Dwarf Viruses

Abstract

Monoclonal antibodies of hybridoma clones derived from mice injected with either the RPV or MAV isolate of barley yellow dwarf virus (BYDV) were evaluated in a variety of tests with 5 previously characterized BYDV luteoviruses. Five cell lines were from mice injected with RPV, and 8 lines were from MAV-injected mice. Three of the 13 cloned hybridomas ceased producing antibodies; 3 others produced antibodies that reacted with healthy oat antigen. Of the 7 virus-specific antibodies, which represented 4 antibody isotypes, 3 reacted only with RPV and one only with MAV. Two additional MAV-derived antibodies reacted with both MAV and PAV. One reacted with MAV and SGV. Specificity of the reactions was consistent in 2 kinds of enzyme-linked immunosorbent assays [ELISA], and in preliminary neutralization studies carried out in test tubes or within aphid vectors. The potential value of these monoclonal antibodies for use in luteovirus research is discussed.