Effect of acute overload on protein synthesis in cardiac muscle microsomes
- 30 November 1967
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Legacy Content
- Vol. 213 (6), 1552-1555
- https://doi.org/10.1152/ajplegacy.1967.213.6.1552
Abstract
Acute overload in the isolated perfused heart results in increased incorporation of labeled amino acids into cardiac protein. Protein synthesis by microsomes, isolated from overloaded guinea pig left ventricles, was studied in a cell-free system using lysine-14c and leucine-14c. Control and overloaded hearts were perfused with ventricular flows of 7 ml/min. for 1-3 hr. with average aortic systolic pressures of 30 mm Hg in controls and 60 mm Hg in the stressed hearts. Microsomes from the overloaded left ventricles incorporated more than twice the amount of lysine-14c or leucine-14c into protein than the controls (515[plus or minus]30 compared to 213[plus or m inus] 10 counts/ min/mg protein N). Cross-incubation studies showed that microsomes from stressed ventricles did not lose their capacity to synthesize protein when incubated in control cell sap. Control microsomes, incubated in cell sap from overloaded ventricles, were not stimulated to increase incorporation of labeled amino acids into protein. Acute cardiac overload stimulates augmented microsomal protein synthesis independent of the source of cell sap or substrate as early as 1 hr. after onset of the stress. The specific part of the microsome affected remains to be clarified.This publication has 14 references indexed in Scilit:
- Polyribosomes and Protein Synthesis in the SpleenJournal of Biological Chemistry, 1966
- Coronary flow rate and perfusion pressure as determinants of mechanical function and oxidative metabolism of isolated perfused rat heart.The Journal of Physiology, 1965
- Amino Acid Incorporation into Protein by Cell-free Systems from Rat Skeletal Muscle. IV. Effects of Animal Age, Androgens, and Anabolic Agents on Activity of Muscle Ribosomes*Biochemistry, 1965
- THE ISOLATION AND PROPERTIES OF CARDIAC RIBOSOMES AND POLYSOMESBiochemical Journal, 1965
- Amino Acid Incorporation into Protein by Cell-free Preparations from Rat Skeletal Muscle. III. Comparisons of Activity of Muscle and Liver RibosomesBiochemistry, 1965
- Studies on the nature of polysomesBiochemical Journal, 1964
- Protein metabolism in cardiac hypertrophy and heart failureAmerican Journal of Physiology-Legacy Content, 1964
- Carbon Tetrachloride Poisoning in Rats: Alteration in Ribosomes of the LiverScience, 1963
- Nucleic Acid Studies in Experimental CardiomegalyCirculation Research, 1963
- A SOLUBLE RIBONUCLEIC ACID INTERMEDIATE IN PROTEIN SYNTHESISJournal of Biological Chemistry, 1958