The enzymic estimation of glutamate and glutamine
- 1 April 1966
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 99 (1), 246-252
- https://doi.org/10.1042/bj0990246
Abstract
A method of estimating glutamic acid is described, based on its dehydrogenation by glutamate dehydrogenase coupled, by means of N-methylphenazine methosulphate, to the reduction of tetrazolium salts. The method is suitable for the estimation of 0-0.3 [mu]mole of glutamic acid. The response is linear, but not stoichiometric: possible reasons for this are discussed. If suitable precautions are taken, the use of a partially purified preparation of glutaminase makes it possible to estimate glutamine also.This publication has 13 references indexed in Scilit:
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