l- andd-alanine transport in brush border membrane vesicles from lepidopteran midgut: Evidence for two transport systems
- 1 October 1984
- journal article
- research article
- Published by Springer Nature in The Journal of Membrane Biology
- Vol. 81 (3), 233-240
- https://doi.org/10.1007/bf01868716
Abstract
In brush border membrane vesicles from the midgut ofPhilosamia cynthia larvae (Lepidoptera) thel- andd-alanine uptake is dependent on a potassium gradient and on transmembrane electrical potential difference. Each isomer inhibits the uptake of the other form: inhibition ofl-alanine uptake byd-alanine is competitive, whereas inhibition ofd-alanine uptake byl-alanine is noncompetitive. Transstimulation experiments as well as the different pattern of specificity to cations suggest the existence of two transport systems. Kinetic parameters for the two transporters have been calculated both when Kout>Kin and Kout=Kin.d-alanine is actively transported also by the whole midgut, but it is not metabolized by the intestinal tissue.This publication has 18 references indexed in Scilit:
- Intestinal amino acid absorption in lepidopteran larvaeBiochimica et Biophysica Acta (BBA) - Biomembranes, 1982
- Nutritive effects of d-amino acids on the silkworm, Bombyx moriJournal of Insect Physiology, 1981
- K+-dependent phenylalanine uptake in membrane vesicels isolated from the midgut of Philosamia cynthia larvaeBiochimica et Biophysica Acta (BBA) - Biomembranes, 1980
- The morphology and fine structure of the larval midgut of a moth (Manduca sexta) in relation to active ion transportTissue and Cell, 1979
- Cellular ionic concentrations in the midgut of two larvae of lepidoptera in vivo and in vitroComparative Biochemistry and Physiology Part A: Physiology, 1978
- A modified procedure for the rapid preparation of efficiently transporting vesicles from small intestinal brush border membranes. Their use in investigating some properties of d-glucose and choline transport systemsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1978
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Biochemical, genetic, and regulatory studies of alanine catabolism in Escherichia coli K12Molecular Genetics and Genomics, 1976
- Purification of the human intestinal brush border membraneBiochimica et Biophysica Acta (BBA) - Biomembranes, 1973