Granulocytes and Phorbol Myristate Acetate Increase Permeability to Albumin of Cultured Endothelial Monolayers and Isolated Perfused Lungs
- 1 January 1983
- journal article
- research article
- Published by American Thoracic Society in American Review of Respiratory Disease
- Vol. 127 (1), 72-76
- https://doi.org/10.1164/arrd.1983.127.1.72
Abstract
Human granulocytes and phorbol myristate acetate (PMA) increased permeability to albumin of monolayers of cultured endothelial cells grown on micropore filters. Granulocytes from a patient with chronic granulomatous disease and PMA did not increase endothelial permeability to albumin, demonstrating that the increase in permeability is dependent on granulocyte-derived oxygen radicals. When granulocytes were separated from the endothelial cells by a micropore filter, granulocytes and PMA no longer increased endothelial permeability to albumin, demonstrating that PMA-stimulated granulocytes must be closely approximated to endothelial cells to increase endothelial permeability. The relevance of these in vitro findings to an intact microvasculature was confirmed by demonstrating that agents that reduce granulocyte adherence to endothelium reduce edema formed in isolated lungs by granulocytes and PMA, an oxygen radical dependent process. Pretreatment of granulocytes with cytochalasin B or addition of 2% dextran to isolated lung perfusates reduced granulocyte adherence and markedly reduced edema formation in isolated lungs. These studies demonstrate that PMA-stimulated granulocytes must be closely apposed to endothelial cells to increase endothelial permeability through an oxygen-radical-dependent mechanism, and they suggest that reduction of granulocyte adherence may protect against granulocyte-dependent edema.This publication has 17 references indexed in Scilit:
- Neutrophil-endothelial cell interactions on endothelial monolayers grown on micropore filters.JCI Insight, 1981
- Thromboxane A2 Mediates Augmented Polymorphonuclear Leukocyte AdhesivenessJCI Insight, 1980
- Role of Complement Activation in a Model of Adult Respiratory Distress SyndromeJCI Insight, 1980
- AN OXYGEN-DEPENDENT MECHANISM OF NEUTROPHIL-MEDIATED CYTO-TOXICITY1980
- EFFECT OF GRANULOCYTOPENIA ON EXTRA-VASCULAR LUNG WATER-CONTENT AFTER MICROEMBOLIZATIONPublished by Elsevier ,1980
- Polymorphonuclear Leukocyte-Mediated, Antibody-Dependent, Cellular Cytotoxicity against Tumor Cells: Dependence on Oxygen and the Respiratory BurstThe Journal of Immunology, 1979
- Extracellular cytolysis by activated macrophages and granulocytes. II. Hydrogen peroxide as a mediator of cytotoxicity.The Journal of Experimental Medicine, 1979
- Selection and characterization of bovine aortic endothelial cellsIn Vitro Cellular & Developmental Biology - Plant, 1978
- Oxygen radicals mediate endothelial cell damage by complement-stimulated granulocytes. An in vitro model of immune vascular damage.JCI Insight, 1978
- The chemotactic attraction of human fibroblasts to a lymphocyte-derived factor.The Journal of Experimental Medicine, 1976