Isolation of Escherichia coli mutants with an adenosine triphosphatase insensitive to aurovertin
- 1 April 1978
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 134 (1), 17-23
- https://doi.org/10.1128/jb.134.1.17-23.1978
Abstract
Energy-transducing ATPase from E. coli is inhibited by aurovertin. Aurovertin-resistant mutants were generated by nitrosoguanidine mutagenesis of E. coli AN180, whose growth on a nonfermentable C source was blocked by aurovertin. The ATPase activity of cell extracts from 15 different mutants (designated MA1, MA2, MA3, etc.) was at least 20 times less sensitive to aurovertin than that from the parent strain. The aurovertin-resistant mutants did not show cross-resistance towards a number of ATPase inhibitors including azide, dicyclohexylcarbodiimide, quercetin, 7-chloro-4-nitrobenzofurazan and N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline. Aurovertin inhibited the energization brought about by addition of ATP to E. coli AN180 membrane vesicles; it was without effect on MA1 and MA2 membrane vesicles energized by ATP. The mutation in MA1, like other mutations of the ATPase complex, maps in the unc region of the bacterial chromosome.This publication has 38 references indexed in Scilit:
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