Interaction of diet and toxicity ? the future role of purified diet in toxicological research

Abstract

There is a growing awareness of the importance of diet as a determinant of the toxicity of many compounds. This paper briefly reviews some of the ways in which diet affects toxicity, and draws some conclusions regarding the ideal diet for toxicological investigations. Then the two competing types of diet, stock and purified (including those frequently called semi-synthetic or semi-purified), are described and their characteristic strengths and weaknesses are discussed. Stock diets are very variable commodities, may be nutritionally poorly balanced, and also contain many non-nutritive components that influence toxicity. The formulation and preparation of purified diet are discussed. It is concluded that investigations of the mechanisms of toxicity, as well as studies of absorption, distribution and metabolism of toxic compounds, could benefit from the use of well-defined purified diets.