Reversed-Phase Cleanup and High Performance Liquid Chromatographic Analysis of Abscisic Acid inCercospora rosicola, Liquid Culture Media
- 1 January 1982
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 5 (1), 81-91
- https://doi.org/10.1080/01483918208068821
Abstract
A high performance liquid chromatographic method is described for measuring abscisic acid produced by the mold Cercospora rosicola Passerini in liquid culture media. The acidified liquid medium was passed through a Cis reversed-phase cartridge, impurities and ABA were eluted separately, and the ABA fraction was chromatographed on a reversed-phase column. Detection was by UV absorbance at 268 nm. No significant difference was found between ABA recoveries obtained by solvent partition and reversed-phase cartridge cleanup.This publication has 10 references indexed in Scilit:
- Derivatization of abscisic acid as the p-nitrobenzyl esterJournal of Agricultural and Food Chemistry, 1978
- Analysis of abscisins and 3-indolylacetic acid in leaves of Sorghum bicolor by high performance liquid chromatographyCanadian Journal of Botany, 1978
- (+)-Abscisic acid, a metabolite of the fungusCercospora rosicolaCellular and Molecular Life Sciences, 1977
- Pro experimentisCellular and Molecular Life Sciences, 1977
- Rapid Separation and Quantification of Abscisic Acid from Plant Tissues Using High Performance Liquid ChromatographyPlant Physiology, 1977
- Screening of the genus Cercospora for secondary metabolites☆Phytochemistry, 1977
- High-performance liquid chromatographic analysis of abscisic acid in plant extractsAnalytical Biochemistry, 1976
- Abscisic Acid Analysis in Vitis vinifera in the Period of Endogenous Bud Dormancy by High Pressure Liquid ChromatographyPhysiologia Plantarum, 1975