Nucleotide sequence and chromosomal assignment of a cDNA encoding the large isoform of human glutamate decarboxylase
- 1 July 1992
- journal article
- Published by Wiley in Annals of Human Genetics
- Vol. 56 (3), 255-265
- https://doi.org/10.1111/j.1469-1809.1992.tb01150.x
Abstract
Glutamic acid decarboxylase (GAD) catalyses the conversion of L-glutamic acid to the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). Two forms of human GAD, GAD65 and GAD67, are encoded by two separate genes. A full length human GAD67 cDNA has been isolated from a human frontal cortex cDNA library and the nucleotide sequence determined. The GAD67 gene has been mapped to chromosome 2 using the polymerase chain reaction to amplify specifically the human sequence in rodent/human somatic cell hybrid DNA. This confirms that human GAD67 is not syntenic with the smaller GAD isoform GAD65 which has been assigned to chromosome 10. Production of polyclonal antiserum to a baculovirus-expressed GAD67 enabled immunocytological detection of GAD in the rat brain.Keywords
This publication has 28 references indexed in Scilit:
- Cloning and partial nucleotide sequence of human glutamic acid decarboxylase cDNA from brain and pancreatic isletsBiochemical and Biophysical Research Communications, 1991
- Sequence of a novel carbonic anhydrase‐related polypeptide and its exclusive presence in purkinje cellsFEBS Letters, 1990
- Identification of the 64K autoantigen in insulin-dependent diabetes as the GABA-synthesizing enzyme glutamic acid decarboxylaseNature, 1990
- Autoantibodies to GABA-ergic Neurons and Pancreatic Beta Cells in Stiff-Man SyndromeNew England Journal of Medicine, 1990
- Molecular Identification of the 62 kd Form of Glutamic Acid Decarboxylase from the MouseEuropean Journal of Neuroscience, 1990
- Drosophila GABAergic Systems: Sequence and Expression of Glutamic Acid DecarboxylaseJournal of Neurochemistry, 1990
- The gene for human muscle-specific phosphoglycerate mutase, PGAM2, mapped to chromosome 7 by polymerase chain reactionGenomics, 1989
- Characterization of a panel of highly variable minisatellites cloned from human DNAAnnals of Human Genetics, 1987
- Molecular cloning, expression and in situ hybridization of rat brain glutamic acid decarboxylase messenger RNANeuroscience Letters, 1987
- The chymotryptic phosphopyridoxyl peptide of DOPA decarboxylase from pig kidneyBiochemical and Biophysical Research Communications, 1977