Antibodies specific for left-handed Z-DNA.
- 1 June 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (6), 3546-3550
- https://doi.org/10.1073/pnas.78.6.3546
Abstract
We prepared a brominated poly(dG-dC).poly(dG-dC) which forms a stable Z-DNA helix under physiological salt conditions. Rabbits and mice were immunized with brominated and unbrominated poly(dG-dC).poly(dG-dC) complexed with methylated bovine serum albumin. Antibodies specific for Z-DNA were produced. These antibodies were found not only in the sera of animals immunized with the low-salt stabilized Z-DNA [Br-poly(dG-dC).poly(dG-dC)] but also in sera from animals immunized with the unbrominated B-DNA form of the polymer. From this it is inferred that the unbrominated poly(dG-dC).poly(dG-dC) was partially converted to Z-DNA by its combination with the basic protein methylated bovine serum albumin. In addition to specific anti-Z-DNA antibody populations, two other interesting types of antibody populations were found. One of these reacted with both the Z and B forms of poly(dG-dC).poly(dG-dC). This antibody may be converting the polymer from the B-DNA to the Z-DNA form. The other type of antibody was specific for a B form of poly(dG-dC).poly(dG-dC) and did not react at all with the Z form. The antibodies raised to Z-DNA were shown to be highly specific for Z-DNA and did not react with B-DNA, RNA, DNA.RNA hybrids, or a number of other polynucleotides. This specificity for Z-DNA will make possible their use as reagents for determining the presence of Z-DNA in biological systems. Sera of autoimmune lupus mice were also shown to have a considerable amount of naturally occurring anti-Z-DNA antibody activity.This publication has 15 references indexed in Scilit:
- Induction of the Z conformation in poly(dG-dC).poly(dG-dC) by binding of N-2-acetylaminofluorene to guanine residues.Proceedings of the National Academy of Sciences, 1981
- Molecular structure of a left-handed double helical DNA fragment at atomic resolutionNature, 1979
- A hypothesis on a specific sequence-dependent conformation of DNA and its relation to the binding of the lac-repressor proteinJournal of Molecular Biology, 1979
- Further studies on the interaction between polynucleotides and antibodies to poly(inosinic acid)·poly(cytidylic acid)Biochemistry, 1978
- Immunofluorescent Detection of Nuclear Double-Stranded RNA in Situ in Vero and Mosquito CellsScience, 1978
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- High resolution detection of DNA–RNA hybrids in situ by indirect immunofluorescenceNature, 1977
- The Specificity and Applications of Antibodies to Helical Nucleic AcidCRC Critical Reviews in Biochemistry, 1975
- Antibodies recognise specific structures of triple-helical polynucleotides built on poly(A) or poly(dA)Nature, 1974
- Salt-induced co-operative conformational change of a synthetic DNA: Equilibrium and kinetic studies with poly(dG-dC)Journal of Molecular Biology, 1972