Cytochrome c Peroxidase. 1. Preparation of the Crystalline Enzyme from Baker's Yeast.

Abstract

A procedure for the purification of cytochrome c peroxidase from baker''s yeast has been described. This method was found to be reproducible and has consistently given a homogeneous crystalline preparation, the iron content of which has been equal to 0.15% per unit of dry weight corresponding to a minimal molecular weight of 37000. The enzyme was found to be free from carbohydrates and to behave as a single homogeneous component in the ultracentrifuge and in free electrophoresis. The isoelectric point of crystalline cytochrome c peroxidase was found to be equal to 4.9 at an ionic strength of 0.1 at + 2[degree]C. The enzyme was stable between pH values of 3.5 and 9.0 at room temperature.