Production of Lantipeptides in Escherichia coli
Open Access
- 29 November 2010
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 133 (8), 2338-2341
- https://doi.org/10.1021/ja109044r
Abstract
Lantipeptides are ribosomally synthesized and posttranslationally modified peptides containing thioether cross-links. We describe the preparation of seven different lantipeptides in Escherichia coli and demonstrate that this methodology can be used to incorporate nonproteinogenic amino acids.Keywords
This publication has 17 references indexed in Scilit:
- Catalytic promiscuity in the biosynthesis of cyclic peptide secondary metabolites in planktonic marine cyanobacteriaProceedings of the National Academy of Sciences, 2010
- Discovery of Unique Lanthionine Synthetases Reveals New Mechanistic and Evolutionary InsightsPLoS Biology, 2010
- Follow the leader: the use of leader peptides to guide natural product biosynthesisNature Chemical Biology, 2009
- Insights into the Mode of Action of the Two-Peptide Lantibiotic HaloduracinACS Chemical Biology, 2009
- In Vitro Mutasynthesis of Lantibiotic Analogues Containing Nonproteinogenic Amino AcidsJournal of the American Chemical Society, 2009
- Chapter 22 Whole‐Cell Generation of Lantibiotic VariantsMethods in Enzymology, 2009
- Chapter 21 In Vitro Studies of Lantibiotic BiosynthesisMethods in Enzymology, 2009
- Lantibiotics: Peptides of Diverse Structure and FunctionAnnual Review of Microbiology, 2007
- Circumventing the Effect of Product Toxicity: Development of a Novel Two-Stage Production Process for the Lantibiotic GalliderminApplied and Environmental Microbiology, 2007
- Discovery and in vitro biosynthesis of haloduracin, a two-component lantibioticProceedings of the National Academy of Sciences, 2006