Mini-collagens in hydra nematocytes.
Open Access
- 15 November 1991
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 115 (4), 1159-1169
- https://doi.org/10.1083/jcb.115.4.1159
Abstract
We have isolated and characterized four collagen-related c-DNA clones (N-COL 1, N-COL 2, N-COL 3, N-COL 4) that are highly expressed in developing nematocytes in hydra. All four c-DNAs as well as their corresponding transcripts are small in size (600-1,000 bp). The deduced amino acid sequences show that they contain a central region consisting of 14 to 16 Gly-X-Y triplets. This region is flanked amino-terminal by a stretch of 14-23 proline residues and carboxy-terminal by a stretch of 6-9 prolines. At the NH2- and COOH-termini are repeated patterns of cysteine residues that are highly conserved between the molecules. A model is proposed which consists of a central stable collagen triple helix of 12-14 nm length from which three 9-22 nm long polyproline II type helices emerge at both ends. Disulfide linkage between cysteine-rich segments in these helices could lead to the formation of oligomeric network structures. Electrophoretic characterization of nematocyst extracts allows resolution of small proline-rich polypeptides that correspond in size to the cloned sequences.Keywords
This publication has 27 references indexed in Scilit:
- Cloning and sequencing of a Porifera partial cDNA coding for a short‐chain collagenEuropean Journal of Biochemistry, 1990
- The extracellular matrix of Volvox carteri: molecular structure of the cellular compartment.The Journal of cell biology, 1989
- Patterns of Amino Acids near Signal‐Sequence Cleavage SitesEuropean Journal of Biochemistry, 1983
- Comparisons of the complete sequences of two collagen genes from Caenorhabditis elegansCell, 1982
- The morphogenesis of nematocytes in Hydra and Forsklia: An ultrastructural studyJournal of Ultrastructure Research, 1981
- Crystallographic refinement and atomic models of the intact immunoglobulin molecule Kol and its antigen-binding fragment at 3.0 Å and 1.9 Å resolutionJournal of Molecular Biology, 1980
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- Isolation of galactose-inducible DNA sequences from Saccharomyces cerevisiae by differential plaque filter hybridizationCell, 1979
- The Development of the Cnidoblasts of HydraThe Journal of cell biology, 1959
- A hydroxyproline-rich, intracellular, collagen-like protein of Hydra nematocystsBiochimica et Biophysica Acta, 1957