Determination of Ofloxacin in Pharmaceutical Forms by High - Performance Liquid Chromatography and Derivative Uvspectrophotometry
- 1 October 1993
- journal article
- pharmaceutical analysis
- Published by Taylor & Francis in Analytical Letters
- Vol. 26 (10), 2193-2201
- https://doi.org/10.1080/00032719308017462
Abstract
A method for the determination of ofloxacin in pharmaceutical forms is described. The procedure is based on the use of the high-performance liquid chromatography, and of the second-derivative ultraviolet spectra, by utilizing the linear relationship between substance concentration and derivative peak amplitude. The minimum concentration detectable by derivative spectrophotometry was 20 ng/ml, and by HPLC 10 ng/ml. The proposed methods, which give thoroughly comparable data, are simple and rapid, and allow precise and accurate results.Keywords
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