Sheep liver serine-threonine dehydratase. I. Purification and evidence for covalently linked α-ketobutyrate as its cofactor
- 23 September 1975
- journal article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 14 (19), 4273-4276
- https://doi.org/10.1021/bi00690a020
Abstract
L-Serine-threonine dehydratase (EC 4.2.1.16) from sheep liver has been obtained as a highly purified preparation as shown by ultracentrifuge studies and analytical disc gel electrophoresis. The dehydratase has a molecular weight of 98,000 +/- 10,000 and is composed of two nonidentical subunits with molecular weights of 41,000 and 47,000. The 41,000 subunit is covalently linked to the carbonyl reagent-sensitive coenzyme which has been identified as alpha-ketobutyric acid.Keywords
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